Journal: Kidney international

Article Title: Adipose tissue explants and MDCK cells reciprocally regulate their morphogenesis in coculture.

doi: 10.1038/ki.2010.68

Figure Lengend Snippet: Figure 8 | Adipokine treatment. Effects of leptin (b–e), its antibody (insets in b–e), and combination of leptin and adiponection (f) on morphology of Madin–Darby canine kidney (MDCK) cells at 7 days in culture. (a) (Control), MDCK cells without leptin and adiponectin treatment show a flat shape with cytoplasm and flattened nuclei. (b and c) 20 (b) or 50 ng/ml (c) leptin does not affect the morphology of MDCK cells. (d and e) 100 (d) or 1000 ng/ml (e) leptin allows MDCK cells to develop a tall and large columnar shape, with clear cytoplasm and vertically and basally situated nuclei. The leptin-induced morphology is similar to the adipose tissue fragment (ATF)- induced morphology of MDCK cells. Leptin antibody (L-ab, 25 mg/ml) abolishes the MDCK cell morphology induced by 100 (inset in d) or 1000 ng/ml leptin (inset in e), although the antibody does not affect the morphology of cells at the concentration of 20 (inset in b) or 50 ng/ml (inset in c) leptin. (f) Adiponectin (10 mg/ml) abolishes the MDCK cell morphology induced by 1000 ng/ml leptin.

Article Snippet: To examine the specificity of a leptin-induced effect on the morphology of MDCK cells, 25 mg/ml of rabbit polyclonal leptin antibody against rat leptin (BioVendor LLC, Candler, NC, USA) was added to the 20, 50, 100, or 1000 ng/ml leptin-treated cultures of MDCK cells alone.

Techniques: Control, Concentration Assay

Journal: Kidney international

Article Title: Adipose tissue explants and MDCK cells reciprocally regulate their morphogenesis in coculture.

doi: 10.1038/ki.2010.68

Figure Lengend Snippet: Figure 9 | Adiponectin and leptin production of adipose tissue fragments (ATFs) in the absence or presence of Madin–Darby canine kidney (MDCK) cells in the supernatants of outer and inner dishes at 7 days in culture. In the cultures of ATFs alone, adiponectin and leptin are detected. Their concentration is not significantly different between the outer and inner dishes (P40.05). In the supernatants of outer dishes, adiponectin concentrations in the cocultures of ATFs and MDCK cells are significantly higher than those in the cultures of ATFs alone (Po0.05), whereas leptin concentrations are not significantly different between cultures of ATFs with and without MDCK cells (P40.05). In cocultures of ATFs and MDCK cells, adiponectin (Po0.01) and leptin (Po0.05) concentrations in the inner dishes were significantly lower than those in the outer dishes.

Article Snippet: To examine the specificity of a leptin-induced effect on the morphology of MDCK cells, 25 mg/ml of rabbit polyclonal leptin antibody against rat leptin (BioVendor LLC, Candler, NC, USA) was added to the 20, 50, 100, or 1000 ng/ml leptin-treated cultures of MDCK cells alone.

Techniques: Concentration Assay

Journal:

Article Title: Phospholipase D2 Is Localized to the Rims of the Golgi Apparatus in Mammalian Cells

doi: 10.1091/mbc.02-04-0059

Figure Lengend Snippet: PLD2 localization with different organelles in rat NRK cells. Cells were prepared for immunofluorescence microscopy and incubated with rabbit PLD2 antibody PLD2-27 (A, D, G, and J) and costained with monoclonal antibodies to the ER marker BiP (B); the transferrin receptor (E), a marker of early endosomes and the plasma membrane; and lgp120 (H), a late endosome/lysosomal marker. (K) Cells were stained with caveolin-1, a marker for caveoli; the arrow corresponds to caveolin-1 localized to the plasma membrane. Images were merged to determine overlap between PLD2 (red) and the respective marker proteins (green; C, F, I, and L). Areas of maximal overlap are yellow. (L) Inset, enlargement of the region of overlap between PLD2 and caveolin-1 in the perinuclear Golgi apparatus. The arrow indicates areas where PLD2 puncta (red) either overlap completely with caveolin-1 (green) or are directly adjacent. Images are from projected Z-series. Bars, 10 μm.

Article Snippet: Purified monoclonal anti-rat transferrin receptor antibodies were purchased from Cedarlane Laboratories (Hornby, Ontario, Canada).

Techniques: Immunofluorescence, Microscopy, Incubation, Marker, Staining

Journal: Journal of Neuroinflammation

Article Title: Involvement of neuronal IL-1β in acquired brain lesions in a rat model of neonatal encephalopathy

doi: 10.1186/1742-2094-10-110

Figure Lengend Snippet: List and features of antibodies

Article Snippet: Anti-PMN , Cedarlane - CLAD51140 , 1:200.

Techniques: